Application
Formalin fixed paraffin embedded human skin stained with Cytokeratin 8/18 antibody .| Product Name | Cytokeratin 8 + 18 antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [5D3] to Cytokeratin 8+18 predilutedKeratin 8 belongs to the type B (basic) subfamily of high molecular weight |
| Immunogen | Human breast carcinoma cell line MCF-7. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5D3 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| This antibody has been pretitered and quality controlled to work allon formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | 3856 CYK8 KRT18 148060 Cytokeratin 8 + 18 KRT8 K18 CYK18 Cytokeratin8 + 18 KO P05787 K8 P05783 K2C8 CK8 CARD2 3875 148070 |
|---|---|
| Target | Cytokeratin 8 + 18 |
| Tissue Specificity | This antibody is specific to human cytokeratins 8 (52.5 kDa) and 18 (45 kDa). This antibody stains simple and glandular epithelium. This antibody can be useful for the identification of adenocarcinomas and most squamous cell carcinomas. |
Application
Formalin fixed paraffin embedded human skin stained with Cytokeratin 8/18 antibody .| Application Notes | This antibody has been pretitered and quality controlled to work allon formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | PBS with sodium azide |
