Application
Immunohistochemical staining of paraffin-embedded human colon tissue using anti-KRT8 mouse monoclonal antibody.Application
Anti-KRT8 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with KRT8Application
Immunofluorescent staining of HeLa cells using anti-KRT8 mouse monoclonal antibody .Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8.Application
Immunofluorescent staining of A549 cells using anti-KRT8 mouse monoclonal antibody .Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded human breast tissue using anti-KRT8 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded human ovary tissue using anti-KRT8 mouse monoclonal antibody.| Product Name | Cytokeratin 8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [1B12] to Cytokeratin 8Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. |
| Immunogen | Recombinant fragment expressed in E.coli corresponding to amino acids 91-381 of human CK8 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 1B12 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| ICC/IF (1:100): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KRT8 K2C8 CYK8 KO K8 CK8 CARD2 |
|---|---|
| Target | Cytokeratin 8 |
| Tissue Specificity | is specific for amino acids 91-381 of human CK8 |
Application
Immunohistochemical staining of paraffin-embedded human colon tissue using anti-KRT8 mouse monoclonal antibody.
Application
Anti-KRT8 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with KRT8
Application
Immunofluorescent staining of HeLa cells using anti-KRT8 mouse monoclonal antibody .
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8.
Application
Immunofluorescent staining of A549 cells using anti-KRT8 mouse monoclonal antibody .
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded human breast tissue using anti-KRT8 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded human ovary tissue using anti-KRT8 mouse monoclonal antibody.| Application Notes | ICC/IF (1:100): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
