Application
Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 8 antibody (cat. # ).| Product Name | Cytokeratin 8 antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [35bE11] to Cytokeratin 8 predilutedKeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18. |
| Immunogen | BALB/C mice were immunized with cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 35bE11 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| IHC-P: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions alland the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | Cytokeratin8 Cytokeratin 8 3856 CYK8 148060 KRT8 KO P05787 K8 K2C8 CK8 CARD2 |
|---|---|
| Target | Cytokeratin 8 |
| Tissue Specificity | This antibody recognizes keratin polypeptide of 54 kD. It stains strongly with non-squamous epithelium but does not stain squamous epithelium. It reacts with the sweat gland and ducts in the skin. Adenocarcinomas of the ovary, gastrointestinal tract, ductal carcinoma of breast, pancreas and bile ducts, stain positive with this antibody. |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
Application
Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 8 antibody (cat. # ).| Positive Control | Skin. |
|---|---|
| Application Notes | IHC-P: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions alland the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Constituents: PBS |
