Application
Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 8 antibody .Application
Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 8, using ABC and DAB chromagenApplication
Detection of Cytokeratin 8 by IHC in Human Colon Carcinoma| Product Name | Cytokeratin 8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [35bH11] to Cytokeratin 8Keratin 8 belongs to the type B (basic) subfamily of high molecular weight |
| Immunogen | Cytoskeletal extract of allhuman hepatocellular carcinoma cell line, Hep3B |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 35bH11 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-FIHC-P |
| IHC: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to the immunostaining.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Cytokeratin8 Cytokeratin 8 3856 CYK8 148060 KRT8 KO P05787 K8 K2C8 CK8 CARD2 |
|---|---|
| Target | Cytokeratin 8 |
| Molecular Weight(MW) | 54 (note) |
| Tissue Specificity | This antibody recognizes a keratin polypeptide of 54 kDa.It stains strongly with non-squamous epithelium but does not stain squamous epithelium. It reacts with the sweat gland and ducts in the skin. Adenocarcinoma of the ovary, gastrointestinal tract, ductal carcinoma of breast, pancreas and bile ducts stain positive with this antibody. |
| Cellular Localization | Cytoplasmic |
Application
Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 8 antibody .
Application
Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 8, using ABC and DAB chromagen
Application
Detection of Cytokeratin 8 by IHC in Human Colon Carcinoma| Application Notes | IHC: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to the immunostaining.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Tissue culture supernatant containing 0.05% sodium azide |
