Application
Immunohistochemical analysis of paraffin-embedded human Kidney tissues using CK8 mouse mAbApplication
Western blot analysis using anti-CK8 monoclonal antibody against A549 (1), Hela (2), MCF-7 (3), A431 (4) and HepG2 (5) cell lysate.Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma(A), lung cancer(B) and ovarian cancer tissue(C), showing membrane and cytoplasmic localization with DAB staining using anti-CK8 antibody.Application
Confocal immunofluorescence analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using anti-CK8 monoclonal antioby (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.| Product Name | Cytokeratin 8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [8A5D12] to Cytokeratin 8This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] |
| Immunogen | The immunogen corresponding to a region within amino acids 391 and 483 of human Cytokeratin expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 8A5D12 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CK-8 KRT8 CYK8 K2C8 K8 KO CK8 CARD2 |
|---|---|
| Target | Cytokeratin 8 |
| Molecular Weight(MW) | 54 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded human Kidney tissues using CK8 mouse mAb
Application
Western blot analysis using anti-CK8 monoclonal antibody against A549 (1), Hela (2), MCF-7 (3), A431 (4) and HepG2 (5) cell lysate.
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma(A), lung cancer(B) and ovarian cancer tissue(C), showing membrane and cytoplasmic localization with DAB staining using anti-CK8 antibody.
Application
Confocal immunofluorescence analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using anti-CK8 monoclonal antioby (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
