Application
Detection of cytokeratin 8 in HeLa cell lysate by mouse monoclonal antibody| Product Name | Cytokeratin 8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [C - 51] to Cytokeratin 8Keratin 8 belongs to the type B (basic) subfamily of high molecular weight |
| Immunogen | Tissue / cell preparation (Human). Cytoskeleton preparation from HeLa cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | C-51 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PIPWB |
| For ICC: Use at an assay dependant dilution. For IHC-P: Use at an assay dependant dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. For IP: Use at an assay dependant dilution. For WB: Use at an assay dependant dilution. Predicted molecular weight: 52.5 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanPigSheepBovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Cytokeratin8 Cytokeratin 8 3856 CYK8 148060 KRT8 KO P05787 K8 K2C8 CK8 CARD2 |
|---|---|
| Target | Cytokeratin 8 |
| Tissue Specificity | Cytokeratin 8 (52.5kD) |
| Cellular Localization | Cytoplasmic |
Application
Detection of cytokeratin 8 in HeLa cell lysate by mouse monoclonal antibody| Application Notes | For ICC: Use at an assay dependant dilution. For IHC-P: Use at an assay dependant dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. For IP: Use at an assay dependant dilution. For WB: Use at an assay dependant dilution. Predicted molecular weight: 52.5 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 15mM Sodium Azide |
