Application
Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human DACT3 (1:50, ) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)Application
Cell lysates of mouse brain and 293T (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human DACT3 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Product Name | DACT3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2A5] to DACT3DACT3, a member of the DACT (Dpr/Frodo) gene family, plays an important role in a negative regulator of Wnt/-catenin signaling. Unlike other Wnt signaling inhibitors that are silenced by DNA methylation, DACT3 repression is associated with bivalent histone modifications. |
| Immunogen | Recombinant human DACT3 (89-202aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2A5 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCIHC-PWB |
| Western Blot: Use at 1:250 ~ 500.Optimal working concentrations should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC15476 thymus expressed gene3like dapper antagonist of betacatenin homolog3 (Xenopus laevis) dapper antagonist of beta-catenin homolog 3 (Xenopus laevis) dapper antagonist of betacatenin homolog 3 (Xenopus laevis) thymus expressed gene 3like thymus |
|---|---|
| Target | DACT3 |
| Light Chain Type | kappa |
Application
Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human DACT3 (1:50, ) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Application
Cell lysates of mouse brain and 293T (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human DACT3 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Application Notes | Western Blot: Use at 1:250 ~ 500.Optimal working concentrations should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
