Application
Immunofluorescence of DAD1 in HepG2 cells with DAD1 antibody at 10 ug/mL.Application
Western blot analysis of DAD1 in HepG2 cell lysate with DAD1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.Application
Immunocytochemistry of DAD1 in HepG2 cells with DAD1 antibody at 10 ug/ml.| Product Name | DAD1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DAD1Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death (1), a process important for normal organism development and tissue homeostasis (2). DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity (3,4). Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death (5,6). Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas (7), indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains. |
| Immunogen | Murine DAD1 Peptide |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DAD1 |
|---|---|
| Target | DAD1 |
| Tissue Specificity | This antibody is specific for DAD1 |
Application
Immunofluorescence of DAD1 in HepG2 cells with DAD1 antibody at 10 ug/mL.
Application
Western blot analysis of DAD1 in HepG2 cell lysate with DAD1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Application
Immunocytochemistry of DAD1 in HepG2 cells with DAD1 antibody at 10 ug/ml.| Positive Control | Blocking Peptide: Cat. No. GTX85083-PEP - DAD1 Peptide Lysates: Cat. No. GTX27900 - HepG2 Cell Lysate |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Antibody is supplied in PBS containing 0.02% sodium azide |
