Application
Immunofluorescence of DAD1 in HepG2 cells with DAD1 antibody at 10 μg/mL.Application
Immunocytochemistry of DAD1 in HepG2 cells with DAD1 antibody at 10 μg/mL.Application
Western blot analysis of DAD1 in HepG2 cell lysate with DAD1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Product Name | DAD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | DAD1 Antibody: Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis. DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity. Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death. Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas, indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.1) Nakashima T, Sekiguchi T, Kuraoka A, et al. Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells. Mol. Cell Biol. 1993; 13:6367-74. |
| Immunogen | DAD1 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human DAD1. The immunogen is located within the last 50 amino acids of DAD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| DAD1 antibody can be used for detection of DAD1 by Western blot at 0.5 to 2 μg/mL. Despite its predicted molecular weight, DAD1 migrates at ~22 kDa in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DAD1 |
|---|---|
| Alternative Names | DAD1 Antibody: OST2 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 Defender against cell death 1 Oligosaccharyl transferase subunit DAD1 |
| Sequence Similarities | 2) Cat. No. 17-011 - Hep G2 Cell Slide |
Application
Immunofluorescence of DAD1 in HepG2 cells with DAD1 antibody at 10 μg/mL.
Application
Immunocytochemistry of DAD1 in HepG2 cells with DAD1 antibody at 10 μg/mL.
Application
Western blot analysis of DAD1 in HepG2 cell lysate with DAD1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Application Notes | DAD1 antibody can be used for detection of DAD1 by Western blot at 0.5 to 2 μg/mL. Despite its predicted molecular weight, DAD1 migrates at ~22 kDa in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | DAD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DAD1 Antibody is supplied in PBS containing 0.02% sodium azide. |
