Application
Whole extract of HEK-293 cells overexpressing human DAP Kinase was separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-phospho-DAP-Kinase (pSer308) Clone: DKPS308 . The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293 cells overexpressing human DAP-Kinase (WT) 2. HEK-293 cells overexpressing human DAP-Kinase (mutant S308A)| Product Name | DAP Kinase 1 (phospho Ser308) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [DKPS308] to DAP Kinase 1 (phospho Ser308)Various apoptotic signals use Death Associated Protein Kinase (DAPK) as a downstream effector in different cell types. DAPK is a positive mediator of apoptosis and is widely expressed in many tissues of embryonic and adult origin. DAPK is a Ca2+/calmodulin dependent Ser/Thr kinase that associates with microfilaments. The protein is composed of a multidomain structure. It has a subdomain typical of serine/threonine kinases, a Ca 2+ /calmodulin regulatory domain, eight ankyrin repeats followed by two P-loop motifs and a typical death domain module. It contains two auto-inhibitory domains one of them Ca 2+ /calmodulin dependent. In the absence of this latter domain, DAPK is constitutively active. DAPK activity is also regulated by phosphorylation. DAPK was found to be negatively regulated by autophosphorylation at Ser308 which is in the calmodulin regulatory domain. This autophosphorylation, which occurs in cells at the basal state, lowers the affinity of DAPK for calmodulin and thus the kinase is inactive. Under some apoptotic conditions DAPK undergoes dephosphorylation. As a consequence, it binds to calmodulin with higher affinity, becomes activated, phosphorylates its downstream substrate proteins, and mediates apoptosis. Monoclonal antibodies specific for Phospho DAP-Kinase (pSer308) are important tools for studying the mechanism of DAPK activation in apoptosis. |
| Immunogen | Synthetic peptide, corresponding to amino acids 303-312 (pSER 308 ) of Human DAP Kinase 1. |
| Modification | p-Ser308 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | DKPS308 |
| Host Species | Mouse |
| Tested Applications | ELISAIPWB |
| WB: Use at a concentration of 1 - 2 μg/ml: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 600831 P53355 DAPK DAP Kinase1 1612 DAP Kinase 1 DAPK1 DKFZp781I035 DAP Kinase 1 (phospho S308) DAP Kinase 1 (phospho Ser308) DAP Kinase 1 (pS308) DAP Kinase 1 (pSer308) DAP Kinase 1 phospho S308 DAP Kinase 1 phospho Ser308 Phospho DAP Kinase |
|---|---|
| Target | DAP Kinase 1 (phospho Ser308) |
| Cellular Localization | Cytoplasmic |
Application
Whole extract of HEK-293 cells overexpressing human DAP Kinase was separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-phospho-DAP-Kinase (pSer308) Clone: DKPS308 . The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293 cells overexpressing human DAP-Kinase (WT) 2. HEK-293 cells overexpressing human DAP-Kinase (mutant S308A)| Positive Control | 293T (human embryonal kidney) cells transfected with DAPKinase expression vector |
|---|---|
| Application Notes | WB: Use at a concentration of 1 - 2 μg/ml: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01mM PBS. pH 7.4 |
