Application
Western blot analysis of extracts of various cell lines, using EIF4G2 antibody.Application
Immunohistochemistry of paraffin-embedded human stomach cancer using EIF4G2 antibody at dilution of 1:200 (400x lens).Application
Immunohistochemistry of paraffin-embedded human colon cancer tissue using EIF4G2 antibody.| Product Name | DAP5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DAP5Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic peptide of human EIF4G2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PWB |
| WB: 1:500 - 1:1000. IHC: 1:50 - 1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EIF4G2 eukaryotic translation initiation factor 4 gamma 2 P97 AAG1 NAT1 |
|---|---|
| Target | DAP5 |
Application
Western blot analysis of extracts of various cell lines, using EIF4G2 antibody.
Application
Immunohistochemistry of paraffin-embedded human stomach cancer using EIF4G2 antibody at dilution of 1:200 (400x lens).
Application
Immunohistochemistry of paraffin-embedded human colon cancer tissue using EIF4G2 antibody.| Application Notes | WB: 1:500 - 1:1000. IHC: 1:50 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
