Application
Flow cytometric analysis of Hela cells using DAXX mouse mAb (green) and negative control (purple)Application
Western blot analysis using anti-DAXX mAb against K562 cell lysate (1).Application
Confocal immunofluorescence analysis of PANC-1 cells using anti-DAXX mAb (green). Blue: DRAQ5 fluorescent DNA dye.Application
Confocal immunofluorescence analysis of Hela cells using anti-DAXX mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.| Product Name | Daxx antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [7A11] to DaxxThis gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008] |
| Immunogen | The immunogen corresponding to human DAXX expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7A11 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | BING2 DAP6 DAXX EAP1 |
|---|---|
| Target | Daxx |
| Molecular Weight(MW) | 81 kDa (note) |
Application
Flow cytometric analysis of Hela cells using DAXX mouse mAb (green) and negative control (purple)
Application
Western blot analysis using anti-DAXX mAb against K562 cell lysate (1).
Application
Confocal immunofluorescence analysis of PANC-1 cells using anti-DAXX mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Application
Confocal immunofluorescence analysis of Hela cells using anti-DAXX mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Antibody are purified by protein G affinity chromatography. Liquid in PBS containing 50% glycerol and 0.03% sodium azide. |
