Application
Immunofluorescence staining of Daxx in transfected HeLa human cervix carcinoma cell line. Myc Daxx (green) was stained with anti-human Daxx (DAXX-01) , nuclei were stained with DAPI (blue). 1A - nuclear localization of Daxx in HeLa cells transfected with pCDNA3-MycDaxxApplication
Immunofluorescence staining of Daxx in transfected HeLa human cervix carcinoma cell line. Myc Daxx (green) was stained with anti-human Daxx (DAXX-01) , nuclei were stained with DAPI (blue). 1B - HeLa cells were co-transfected with pCDNA3-MycDaxx and pCDNA3-ASK1HA, which led to translocation of Daxx from nucleus to cytoplasm| Product Name | Daxx antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [DAXX-01] to DaxxApoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx (1,2). Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways (1,2). |
| Immunogen | Recombinant fragment, corresponding to amino acids 558-740 of Human Daxx. Produced in E. coli> |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | DAXX-01 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| ICC: Use at an assay dependant dilution. WB: Use at a dilution of 1/100. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Q9UER7 MGC126246 BING2 1616 MGC126245 DAXX DAP6 603186 |
|---|---|
| Target | Daxx |
Application
Immunofluorescence staining of Daxx in transfected HeLa human cervix carcinoma cell line. Myc Daxx (green) was stained with anti-human Daxx (DAXX-01) , nuclei were stained with DAPI (blue). 1A - nuclear localization of Daxx in HeLa cells transfected with pCDNA3-MycDaxx
Application
Immunofluorescence staining of Daxx in transfected HeLa human cervix carcinoma cell line. Myc Daxx (green) was stained with anti-human Daxx (DAXX-01) , nuclei were stained with DAPI (blue). 1B - HeLa cells were co-transfected with pCDNA3-MycDaxx and pCDNA3-ASK1HA, which led to translocation of Daxx from nucleus to cytoplasm| Positive Control | HeLa whole cell lysate |
|---|---|
| Application Notes | ICC: Use at an assay dependant dilution. WB: Use at a dilution of 1/100. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
