Application
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with DBF4B antibody diluted at 1:500.Application
DBF4B antibody detects DBF4B protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DBF4B protein stained by DBF4B antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | DBF4B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DBF4B (DBF4 homolog B (S. cerevisiae))This gene encodes a regulator of the CDC7-like 1 protein, a serine-threonine kinase which links cell cycle regulation to genome duplication. This protein localizes to the nucleus and its expression is cell cycle-regulated. Alternative splicing of this gene results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full length sequences have not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DBF4B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DBF4 homolog B (S. cerevisiae) ZDBF1B CHIFB DRF1 ASKL1 |
|---|---|
| Target | DBF4B |
| Molecular Weight(MW) | 67 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with DBF4B antibody diluted at 1:500.
Application
DBF4B antibody detects DBF4B protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DBF4B protein stained by DBF4B antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | NT2D1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
