Application
Immunoprecipitation of DCK protein from 293T whole cell extracts using 5 μg of DCK antibody . Western blot analysis was performed using DCK antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
DCK antibody detects DCK protein by western blot analysis. A. 30 µg PC-12 whole cell extract B. 30 µg Rat2 whole cell extract 12% SDS-PAGE DCK antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
DCK antibody detects DCK protein at cytosol and nucleus on human lung carcinoma by immunohistochemical analysis. Sample: DCK antibody dilution: 1:250.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Deoxycytidine kinase antibody at 1:200 dilution.Application
DCK antibody detects DCK protein by western blot analysis. A. 50 µg mouse brain extract 12% SDS-PAGE DCK antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | DCK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DCK (deoxycytidine kinase)Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DCK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (96%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC117410 antibody MGC138632 antibody DCK antibody deoxycytidine kinase antibody deoxynucleoside kinase antibody |
|---|---|
| Molecular Weight(MW) | 31 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Immunoprecipitation of DCK protein from 293T whole cell extracts using 5 μg of DCK antibody . Western blot analysis was performed using DCK antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
DCK antibody detects DCK protein by western blot analysis. A. 30 µg PC-12 whole cell extract B. 30 µg Rat2 whole cell extract 12% SDS-PAGE DCK antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
DCK antibody detects DCK protein at cytosol and nucleus on human lung carcinoma by immunohistochemical analysis. Sample: DCK antibody dilution: 1:250.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Deoxycytidine kinase antibody at 1:200 dilution.
Application
DCK antibody detects DCK protein by western blot analysis. A. 50 µg mouse brain extract 12% SDS-PAGE DCK antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , HepG2 , A549 , mouse brain , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
