Application
WB analysis of indicated lysate using Dynactin 1 antibody at a dilution of 1:1,000.Application
IP analysis of HeLa lysates using control IgG and Dynactin 1 antibody. The precipitates were detected by the same Dynactin 1 antibody.| Product Name | DCTN1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [3D5-C6-D5] to DCTN1This gene encodes the largest subunit of dynactin,a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150kD.Dynactin binds to both microtubules and cytoplasmic dynein.Dynactin is involved in a diverse array of cellular functions,including ER-to-Golgi transport,the centripetal movement of lysosomes and endosomes,spindle formation,chromosome movement,nuclear positioning, and axonogenesis.This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus.Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA).[provided by RefSeq, Oct 2008] |
| Immunogen | Purified recombinant DCTN1 protein fragments expressed in E.coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 3D5-C6-D5 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DAP-150 P135 DP-150 dynactin 1 DCTN1 |
|---|---|
| Target | DCTN1 |
| Molecular Weight(MW) | 150kDa (note) |
| Cellular Localization | Cytoplasm |
Application
WB analysis of indicated lysate using Dynactin 1 antibody at a dilution of 1:1,000.
Application
IP analysis of HeLa lysates using control IgG and Dynactin 1 antibody. The precipitates were detected by the same Dynactin 1 antibody.| Application Notes | IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol. |
