Application
Western blot analysis of extracts of MCF7 cell line, using DCTN2 antibody.Application
Immunofluorescence analysis of U2OS cell using DCTN2 antibody. Blue: DAPI for nuclear staining.Application
Immunohistochemistry of paraffin-embedded human kidney using DCTN2 antibody at dilution of 1:200 (x400 lens)| Product Name | DCTN2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DCTN2This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2012] |
| Immunogen | Recombinant protein of human DCTN2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PIPWB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:20 - 1:50: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | dynactin subunit 2 DCTN2 RBP50 DYNAMITIN DCTN50 |
|---|---|
| Target | DCTN2 |
Application
Western blot analysis of extracts of MCF7 cell line, using DCTN2 antibody.
Application
Immunofluorescence analysis of U2OS cell using DCTN2 antibody. Blue: DAPI for nuclear staining.
Application
Immunohistochemistry of paraffin-embedded human kidney using DCTN2 antibody at dilution of 1:200 (x400 lens)| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:20 - 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
