Application
Confocal immunofluorescent analysis of DDAH1 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Formalin-fixed and paraffin-embedded mouse kidney reacted with DDAH1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry;Application
Western blot analysis of DDAH1 Antibody (C-term) in mouse kidney tissue lysates (35ug/lane). DDAH1 (arrow) was detected using the purified Pab.Application
DDAH1 Antibody (C-term) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DDAH1 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DDAH1This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq, Jul 2008] |
| Immunogen | a KLH conjugated synthetic peptide between 221-250 amino acids from the C-terminal region of human DDAH1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICC/IFIHC-PWB |
| IHC-P: 1:50 - 1:100, WB: 1:1000, FACS: 1:10 - 1:50, ICC/IF: 1:10 - 1:50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Synonyms | dimethylarginine dimethylaminohydrolase 1 DDAH |
|---|---|
| Target | DDAH1 |
| Tissue Specificity | This DDAH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-250 amino acids from the C-terminal region of human DDAH1. |
Application
Confocal immunofluorescent analysis of DDAH1 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Formalin-fixed and paraffin-embedded mouse kidney reacted with DDAH1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry;
Application
Western blot analysis of DDAH1 Antibody (C-term) in mouse kidney tissue lysates (35ug/lane). DDAH1 (arrow) was detected using the purified Pab.
Application
DDAH1 Antibody (C-term) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | IHC-P: 1:50 - 1:100, WB: 1:1000, FACS: 1:10 - 1:50, ICC/IF: 1:10 - 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
