Application
DDB1 antibody detects DDB1 protein at nucleus and cytosol on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. DDB1 antibody dilution: 1:500.Application
Rat tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
DDB1 antibody detects DDB1 protein at nucleus and cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. DDB1 antibody dilution: 1:500.Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
DDB1 antibody immunoprecipitates DDB1 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of DDB1 protein by 2.5 μg of DDB1 antibody 7.5% SDS-PAGE The immunoprecipitated DDB1 protein was detected by DDB1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using DDB1 antibody at 1:500 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDB1 antibody at 1:200 dilution.| Product Name | DDB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DDB1 (damage-specific DNA binding protein 1, 127kDa)This gene encodes the large subunit of DNA damage-binding protein which is a heterodimer composed of a large and a small subunit. This protein functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human DDB1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBIB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Xenopus Tropicalis, Chicken, BovineXenopus laevis (96%), Xenopus Tropicalis (93%), Chicken (98%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DDBA antibody UV-DDB1 antibody XAP1 antibody XPCE antibody XPE antibody XPE-BF antibody DDB1 antibody DNA damage-binding protein a antibody XPE-binding factor antibody UV-DDB 1 antibody xeroderma pigmentosum group E-complementing protein antibody |
|---|---|
| Molecular Weight(MW) | 127 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
DDB1 antibody detects DDB1 protein at nucleus and cytosol on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. DDB1 antibody dilution: 1:500.
Application
Rat tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
DDB1 antibody detects DDB1 protein at nucleus and cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. DDB1 antibody dilution: 1:500.
Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
DDB1 antibody immunoprecipitates DDB1 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of DDB1 protein by 2.5 μg of DDB1 antibody 7.5% SDS-PAGE The immunoprecipitated DDB1 protein was detected by DDB1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using DDB1 antibody at 1:500 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDB1 antibody at 1:200 dilution.| Positive Control | 293T , HepG2 , NIH-3T3 , mouse brain , PC-12 , Rat2 , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
