Application
1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2)| Product Name | DDDDK tag antibody [FG4R] |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Mouse monoclonal antibody [FG4R] to DDDDK tagEpitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins. |
| Immunogen | DYKDDDDK (FLAG) synthetic peptide conjugated to KLH |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | FG4R |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHCIPWB |
| WB (with ECL): Use at a dilution of 1:1000-3000 | |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLAG Tag, DDDDK Tag |
|---|---|
| Target | DDDDK tag |
| Tissue Specificity | Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins |
Application
1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2)| Application Notes | WB (with ECL): Use at a dilution of 1:1000-3000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C (add 0.1% NaN3) for several days to weeks, and at -20°C for longer periods. Avoid repeated freeze-thaw cycles |
| Storage Buffer | 10mM PBS, pH 7.2 |
