Application
Fig. 1. Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. 1A - fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor® 594) 1B - cell nuclei stained with DAPI (blue) 1C - merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue)| Product Name | DDDDK tag antibody [F-tag-01] |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Mouse monoclonal [F-tag-01] to DDDDK tagThis is a useful tool for the localisation and characterisation of DDDDK tagged proteins. |
| Immunogen | Synthetic peptide: DYKDDDK conjugated to KLH. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | F-tag-01 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| ICC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user. | |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | Flag , DDDDK Tag , DDDDDK Tag , DDDDK tag antibody [F tag01] |
|---|---|
| Target | DDDDK tag |
Application
Fig. 1. Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. 1A - fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor® 594) 1B - cell nuclei stained with DAPI (blue) 1C - merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue)| Application Notes | ICC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide. Constituents: PBS. pH 7.4 |
