Application
DDDDK tag antibody [GT231] detects DDDDK-tagged proteins by western blot. A. Untransfected 293T whole cell lysate/extract (30 µg ) B.-D. Whole cell lysate/extracts (30 µg) from transfected 293T cells expressing different DDDDK-tagged proteins. 10% SDS-PAGE DDDDK tag antibody [GT231] dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
DDDDK tag antibody [GT231] immunoprecipitates DDDK-tagged protein in IP experiments. IP Sample: 750 μg whole cell lysate/extract of DDDK-tagged protein expressing 293T cells. A : 5 μg whole cell lysate/extract of DDDDK-tagged protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of DDDK-tagged protein by 2.5 μg of DDDDK tag antibody [GT231] 7.5% SDS-PAGE The immunoprecipitated DDDK-tagged protein was detected by DDDDK tag antibody [GT231] diluted at 1 : 5000. EasyBlot anti-mouse IgG was used as a secondary reagent.| Product Name | DDDDK tag antibody [GT231] |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Mouse Monoclonal antibody [GT231] to DDDDK tag |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT231 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DDDDK tag antibody |
|---|
Application
DDDDK tag antibody [GT231] detects DDDDK-tagged proteins by western blot. A. Untransfected 293T whole cell lysate/extract (30 µg ) B.-D. Whole cell lysate/extracts (30 µg) from transfected 293T cells expressing different DDDDK-tagged proteins. 10% SDS-PAGE DDDDK tag antibody [GT231] dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
DDDDK tag antibody [GT231] immunoprecipitates DDDK-tagged protein in IP experiments. IP Sample: 750 μg whole cell lysate/extract of DDDK-tagged protein expressing 293T cells. A : 5 μg whole cell lysate/extract of DDDDK-tagged protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of DDDK-tagged protein by 2.5 μg of DDDDK tag antibody [GT231] 7.5% SDS-PAGE The immunoprecipitated DDDK-tagged protein was detected by DDDDK tag antibody [GT231] diluted at 1 : 5000. EasyBlot anti-mouse IgG was used as a secondary reagent.| Positive Control | DDDDK-tagged protein expressing 293T cells |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
