Application
Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates with DEDAF antibody at 1μg /ml.Application
Immunohistochemical staining of DEDAF in mouse liver tissue with DEDAF antibody at 10μg/ml.| Product Name | DEDAF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to DEDAFApoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase 8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor. DEDAF is identical to the transcriptional repressor RYBP. DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase 8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis. |
| Immunogen | Synthetic peptide: TPKGDMSAVNDESF, corresponding to amino acids 215-228 of Human DEDAF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| IF: Use at a concentration of 1 μg/ml. WB: Use at a concentration of 0.5 - 1.0 μg/ml. Detects a band of approximately 32 kDa. Can be blocked with GTX26097 DEDAF peptide (human) . Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DEDAF, Q8N488, AAP1, YEAF1, RYBP, 607535, 23429 |
|---|---|
| Target | DEDAF |
Application
Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates with DEDAF antibody at 1μg /ml.
Application
Immunohistochemical staining of DEDAF in mouse liver tissue with DEDAF antibody at 10μg/ml.| Positive Control | A549, HepG2 or 3T3 whole cell lysate. |
|---|---|
| Application Notes | IF: Use at a concentration of 1 μg/ml. WB: Use at a concentration of 0.5 - 1.0 μg/ml. Detects a band of approximately 32 kDa. Can be blocked with GTX26097 DEDAF peptide (human) . Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
