Application
Western blot analysis of DEDAF expression in HepG2 cell lysate| Product Name | DEDAF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to DEDAFCell death signal are transduced by Death Domain (DD), Death Effector Domain (DED), and Caspase Recruitment Domain (CARD) containing molecules. A novel protein that interacts with DED of caspase-8 and -10, and FADD was recently identified and designated DEDAF (for DED associated factor). DEDAF is identical to the transcriptional repressor RYBP. DEDAF interacts with FADD and augments the formation of CD95/FADD/caspase-8 complexes at the cytoplasm. DEDAF also interacts with DED-containing DNA binding protein (DEDD) in the nucleus, indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis. |
| Immunogen | Available on request |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 1.0 μg/ml. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DEDAF, Q8N488, AAP1, YEAF1, RYBP, 607535, 23429 |
|---|---|
| Target | DEDAF |
| Tissue Specificity | This antibody detects ~32kDa DEDAF. |
Application
Western blot analysis of DEDAF expression in HepG2 cell lysate| Positive Control | A549 or HepG2 cell lysate |
|---|---|
| Application Notes | WB: Use at a concentration of 1.0 μg/ml. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide |
