Application
Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] detects Dengue virus 4 protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH. Green: Dengue virus 4 protein stained by Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] detects Dengue virus 3 protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH. Green: Dengue virus 3 protein stained by Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] detects Dengue virus 2 protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH. Green: Dengue virus 2 protein stained by Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] detects Dengue virus 1 protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH. Green: Dengue virus 1 protein stained by Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Dengue virus Type 1, 2, 3 & 4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal to Dengue Virus 1+2+3+4 |
| Immunogen | Mice were immunized with a mixture of dengue 1, 2, 3 and 4. Spleen cells were used to prepare hybridomas and clones selected based on the ability of the antibody they produced to react with each dengue serotype. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | D1-11(3) |
| Host Species | Mouse |
| Tested Applications | DotELISAICC/IFWB |
| For Dot: Use at an assay dependent dilution. For ELISA: Use at an assay dependent dilution. For WB: Use at a concentratio of 1.0 μg/ml. For ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | DengueVirus1 |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Target | Dengue virus 1, 2, 3 & 4 |
|---|---|
| Tissue Specificity | This hybridoma antibody reacts with each dengue serotype in dot blot, ELISA and fluorescent antibody tests. Its reactivity has been confirmed by CDC. |
| Light Chain Type | kappa |
Application
Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] detects Dengue virus 4 protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH. Green: Dengue virus 4 protein stained by Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] detects Dengue virus 3 protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH. Green: Dengue virus 3 protein stained by Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] detects Dengue virus 2 protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH. Green: Dengue virus 2 protein stained by Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] detects Dengue virus 1 protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH. Green: Dengue virus 1 protein stained by Dengue virus 1, 2, 3 & 4 antibody [D1-11(3)] diluted at 1:500. Blue: Hoechst 33342 staining.| Application Notes | For Dot: Use at an assay dependent dilution. For ELISA: Use at an assay dependent dilution. For WB: Use at a concentratio of 1.0 μg/ml. For ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.05% sodium azide |
