Mouse Monoclonal to Human DesminThis antibody reacts with the 53 kD intermediate filament protein desmin in muscle cells. Both striated (skeletal and cardiac) and smooth muscle cells are recognized byThis antibody. The recognition is confined to the Z bands in skeletal and cardiac muscle.
Immunogen
Desmin purified from human muscle
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
33
Host Species
Mouse
Tested Applications
IHC-FIHC-P
Immunohistochemistry (Paraffin/Frozen). Use with AutoProbe III (GTX28620) . For a three-step avidin-biotin complex system with extensive incubation protocols, a dilution of 1:50 to 1:100 may be used as a guideline. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory. Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good diluent to use with most antibodies.
Immunohistochemistry (Paraffin/Frozen). Use with AutoProbe III (GTX28620) . For a three-step avidin-biotin complex system with extensive incubation protocols, a dilution of 1:50 to 1:100 may be used as a guideline. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory. Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good diluent to use with most antibodies.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.
Storage Buffer
20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, and 0.05% Sodium Azide, pH 7.5.