Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-DHFRmouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-DHFR mouse monoclonal antibody. ( Dilution 1:50)Application
Anti-DHFR mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with DHFRApplication
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-DHFRmouse monoclonal antibody. ( Dilution 1:50)Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DHFR monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DHFR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DHFR.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-DHFRmouse monoclonal antibody. ( Dilution 1:50)| Product Name | DHFR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [6G7] to DHFRKey enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. |
| Immunogen | Protein expressed in 293T cell transfected with human DHFR expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6G7 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PWB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanDogMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DYR DHFR DHFRP1 |
|---|---|
| Target | DHFR |
| Tissue Specificity | is specific for human DHFR. |
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-DHFRmouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-DHFR mouse monoclonal antibody. ( Dilution 1:50)
Application
Anti-DHFR mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with DHFR
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-DHFRmouse monoclonal antibody. ( Dilution 1:50)
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DHFR monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DHFR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DHFR.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-DHFRmouse monoclonal antibody. ( Dilution 1:50)| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
