Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Diablo antibody at 1:500 dilution.Application
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Diablo antibody [N1C3] diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:1000| Product Name | Diablo antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Smac (diablo homolog (Drosophila))This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Four alternatively spliced transcript variants have been described for this gene, with two of them encoding different isoforms and the other two probably not encoding a protein. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Diablo. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, BovineRat (84%), Dog (88%), Pig (86%), Bovine (88%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DIABLO-S antibody FLJ10537 antibody FLJ25049 antibody SMAC antibody SMAC3 antibody DIABLO antibody second mitochondria-derived activator of caspase antibody mitochondrial Smac protein antibody 0610041G12Rik antibody direct IAP-binding protein with |
|---|---|
| Molecular Weight(MW) | 27 kDa (note) (2) |
| Cellular Localization | Mitochondrion |
Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Diablo antibody at 1:500 dilution.
Application
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Diablo antibody [N1C3] diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse testis |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
