Application
Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using Diablo antibody at 1:500 dilution.| Product Name | Diablo antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Smac (diablo homolog (Drosophila))This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Four alternatively spliced transcript variants have been described for this gene, with two of them encoding different isoforms and the other two probably not encoding a protein. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Smac. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, BovineMouse (92%), Rat (91%), Dog (92%), Pig (91%), Bovine (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DIABLO-S antibody FLJ10537 antibody FLJ25049 antibody SMAC antibody SMAC3 antibody DIABLO antibody second mitochondria-derived activator of caspase antibody mitochondrial Smac protein antibody 0610041G12Rik antibody direct IAP-binding protein with |
|---|---|
| Molecular Weight(MW) | 27 kDa (note) (3) |
| Cellular Localization | Mitochondrion |
Application
Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using Diablo antibody at 1:500 dilution.| Positive Control | A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
