Application
Western blot analysis of DIO1 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). DIO1 (arrow) was detected using the purified Pab.| Product Name | DIO1 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to DIO1 (N-term)The protein encoded by this gene is a thiol-requiring propylthiouracil-sensitive oxidoreductase. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It also degrades both hormones by inner ring deiodination (IRD). Alternative splicing results in multiple transcript variants encoding different isoforms. Some, but not all, isoforms contain a selenocysteine (Sec) residue encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Additional transcript variants have been described but are not supported by experimental evidence. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 36~66 amino acids from the N-terminal region of human DIO1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TypeI 5'deiodinase ITDI1 Type 1 DI TXDI1 TypeI5'deiodinase Type-I 5'-deiodinase Type1 DI 5DI DIO1 Type I iodothyronine deiodinase DIOI Type I 5' deiodinase |
|---|---|
| Target | DIO1 |
| Molecular Weight(MW) | 28.924 kDa (note) |
Application
Western blot analysis of DIO1 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). DIO1 (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
