Application
DNA polymerase epsilon antibody detects DNA polymerase epsilon protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 15 % SDS-PAGE DNA polymerase epsilon antibody dilution: 1:1000| Product Name | DNA polymerase epsilon antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DNA polymerase epsilon (polymerase (DNA directed), epsilon 3, accessory subunit)POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DNA polymerase epsilon. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, Chimpanzee, BovineMouse (98%), Rat (100%), Zebrafish (87%), Xenopus laevis (95%), Chicken (97%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CHARAC17 YBL1 POLE3 p17 polymerase (DNA directed) epsilon 3 accessory subunit CHRAC17 |
|---|---|
| Target | DNA Polymerase epsilon |
| Molecular Weight(MW) | 17 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
DNA polymerase epsilon antibody detects DNA polymerase epsilon protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 15 % SDS-PAGE DNA polymerase epsilon antibody dilution: 1:1000| Positive Control | 293T , A431 , HeLa , HepG2 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
