| Product Name | DNA polymerase epsilon antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [93H3A] to DNA Polymerase epsilonDNA Polymerase epsilon is a histone fold protein that interacts with other histone fold proteins to bind DNA in a sequence independent manner. These histone fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging. It is present in proliferating cells and is implicated in the repair of UV damaged DNA. It is found to be a component of a recombination complex that is able to repair deletions and doublestrand breaks. |
| Immunogen | Recombinant full length protein, corresponding to amino acids 1-176 of Human DNA Polymerase epsilon. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 93H3A |
| Host Species | Mouse |
| Tested Applications | IPWB |
| For IP (Native and denatured): Use at a concentration of 2μg/mg. For WB: Use at a concentration of 2-4μg/ml for 2hrs at RT. By Western blot, this antibody detects a band of 200kDa identified as DNA polymerase epsilon (polepsilon) and 140kDa polepsilon* Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseMonkeyBovine |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | YBL1 POLE3 p17 CHRAC17 Q9NRF9 DNA polymerase epsilon CHARAC17 54107 607267 |
|---|---|
| Target | DNA polymerase epsilon |
| Tissue Specificity | Detects both pol epsilon and polepsilon* (a form of pol epsilon) It does not cross-react with DNA Polymerase alpha or beta. It does not neutralize the polymerase or exonuclease activity of the enzyme. |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
| Positive Control | 293 cells |
|---|---|
| Application Notes | For IP (Native and denatured): Use at a concentration of 2μg/mg. For WB: Use at a concentration of 2-4μg/ml for 2hrs at RT. By Western blot, this antibody detects a band of 200kDa identified as DNA polymerase epsilon (polepsilon) and 140kDa polepsilon* Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS. pH 7.4 containing 0.2% BSA and 0.09% Sodium azide |
