Application
Western Blot: DNA Polymerase epsilon Antibody (3C5.1) - SDS-polyacrylamide gel analysis of HeLa DNA pol purified by Mono S FPLC. The Mono S fraction of HeLa DNA pol (0.1 mg) was electrophoresed on a 7% SDS-polyacrylamide gel and stained with silver.| Product Name | DNA polymerase epsilon antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal to DNA polymerase epsilonDNA polymerase e from HeLa cells differs from yeast pol e in that HeLa pol e contains two subunits of 220 and 55 kDa, whereas yeast pol e has one large subunit and three smaller subunits. Previous studies of template specificity of HeLa pol e, which appears to be required for long patch DNA repair in permeabilized cells, indicate pol e prefers long stretches of single-stranded regions in poly(dA)-oligo(dT) as template. HeLa pol a and pol d preferactivated DNA and alternating poly(dA-dT) as templates, respectively. Pol e retains its processivity in the absence of proliferating cell nuclear antigen (PCNA), unlike pol d. PCNA can also interact with Pol e on linear DNA templates, even in the absence of other auxiliary factors (replication factor C, replication factor A), and thereby stimulate its primer recognition and DNA synthesis. |
| Immunogen | Mono S FPLC column purified HeLa cell pol epsilon. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 3C5.1 |
| Host Species | Mouse |
| Tested Applications | WB |
| For WB: Use at a concentration of 5 ug/ml. This antibody can be used in Western blot analysis and detects a single band of 220 kDa representing the catalytic subunit of DNA Pol epsilon. Product GTX30112 will immunoprecipitate, but only in the absence of salt and even then poorly. Optimal dilutions/concentrations should be determined by the researcher.: |
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| Species Reactivity | HumanMouseMonkeyHamsterMink |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | YBL1 POLE3 p17 CHRAC17 Q9NRF9 DNA polymerase epsilon CHARAC17 54107 607267 |
|---|---|
| Target | DNA polymerase epsilon |
| Tissue Specificity | This antibody is specific to the human DNA pol epsilon and does not recognize DNA pol alpha, beta or delta. |
Application
Western Blot: DNA Polymerase epsilon Antibody (3C5.1) - SDS-polyacrylamide gel analysis of HeLa DNA pol purified by Mono S FPLC. The Mono S fraction of HeLa DNA pol (0.1 mg) was electrophoresed on a 7% SDS-polyacrylamide gel and stained with silver.| Application Notes | For WB: Use at a concentration of 5 ug/ml. This antibody can be used in Western blot analysis and detects a single band of 220 kDa representing the catalytic subunit of DNA Pol epsilon. Product GTX30112 will immunoprecipitate, but only in the absence of salt and even then poorly. Optimal dilutions/concentrations should be determined by the researcher.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
