Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:1000Application
DNAJC3 antibody detects DNAJC3 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: DNAJC3 protein stained by DNAJC3 antibody diluted at 1:500. Blue: Hoechst 33343 staining.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using DNAJC3 antibody at 1:250 dilution.| Product Name | DNAJC3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DNAJC3 (DnaJ (Hsp40) homolog, subfamily C, member 3)The protein encoded by this gene contains multiple tetratricopeptide repeat (TPR) motifs as well as the highly conserved J domain found in DNAJ chaperone family members. It is a member of the tetratricopeptide repeat family of proteins and acts as an inhibitor of the interferon-induced, dsRNA-activated protein kinase (PKR). [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DNAJC3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Rhesus Monkey, BovineMouse (93%), Rat (92%), Pig (95%), Rhesus Monkey (97%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ21288 antibody HP58 antibody P58 antibody P58IPK antibody PRKRI antibody DNAJC3 antibody protein kinase inhibitor of 58 kDa antibody protein kinase inhibitor p58 antibody interferon-induced double-stranded RNA-activated protein kinase inhibitor |
|---|---|
| Molecular Weight(MW) | 58 kDa (note) |
| Cellular Localization | Endoplasmic reticulum |
Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:1000
Application
DNAJC3 antibody detects DNAJC3 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: DNAJC3 protein stained by DNAJC3 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using DNAJC3 antibody at 1:250 dilution.| Positive Control | Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
