Application
Western blot analysis of Dnmt3a in (A) Dnmt3a transfected and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/ml.Application
Western blot analysis of Dnmt3a using Dnmt3a antibody at 2 ug/ml in 293 cell lysate transfected with either mouse Dnmt3a (lane 1) or mouse Dnmt3b (lane 2).| Product Name | DNMT3A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [64B814] to DNMT3AMethylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt 1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt 1 also cooperates with Rb to repress transcription from promoters containing E2F binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. |
| Immunogen | Bacteria expressed HIS-tag recombinant mouse Dnmt3a. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 64B814 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| IHC: Use at an assay dependent dilution. WB: Use at a concentration of 2 μg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | W91664 1788 DNMT3A2 M.HsaIIIA Q9Y6K1 Dnmt3a 602769 13435 AU015806 |
|---|---|
| Target | Dnmt3a |
| Cellular Localization | Nuclear |
Application
Western blot analysis of Dnmt3a in (A) Dnmt3a transfected and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/ml.
Application
Western blot analysis of Dnmt3a using Dnmt3a antibody at 2 ug/ml in 293 cell lysate transfected with either mouse Dnmt3a (lane 1) or mouse Dnmt3b (lane 2).| Positive Control | 293 cell lysate |
|---|---|
| Application Notes | IHC: Use at an assay dependent dilution. WB: Use at a concentration of 2 μg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
