Application
Western blot analysis of DNMT3L in a human tissue mix using a 1:1000 dilution of| Product Name | DNMT3L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [S117-9] to DNMT3LCpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternative splicing results in two transcript variants. An additional splice variant has been described but its biological validity has not been determined. [provided by RefSeq] |
| Immunogen | Fusion protein amino acids 1-387 of human DNMT3L, accession number BAA95556 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| ICC/IF:1:200~1:500 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | DNA (cytosine5)methyltransferease3like DNA (cytosine-5-)-methyltransferease 3-like MGC1090 DNMT3L |
|---|---|
| Molecular Weight(MW) | 45kDa (note) |
Application
Western blot analysis of DNMT3L in a human tissue mix using a 1:1000 dilution of| Application Notes | ICC/IF:1:200~1:500 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20 C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS pH7.4, 50% glycerol |
