Application
WB analysis of DsbA recombinant protein using DsbA antibody.Application
Western blot detection of DSBA in AKT1,AKT2,AKT3 and DSBA recombinant antigen fragments using DSBA antibody (1:1000 diluted).| Product Name | DsbA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [1A2-D4-G10] to DsbADisulphide oxidoreductase (DsbA) is the major oxidase responsible for generation of disulfide bonds in proteins of E. coli envelope. It is a member of the thioredoxin superfamily. DsbA introduces disulfide bonds directly into substrate proteins by donating the disulfide bond in its active site Cys30-Pro31-His32-Cys33 to a pair of cysteines in substrate proteins. DsbA is reoxidized by dsbB. It is required for pilus biogenesis. |
| Immunogen | Purified recombinant human DsbA protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | E.coli |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | iarA dsbA ppfA JW3832 ECK3852 dsf |
|---|---|
| Molecular Weight(MW) | 24kDa (note) |
Application
WB analysis of DsbA recombinant protein using DsbA antibody.
Application
Western blot detection of DSBA in AKT1,AKT2,AKT3 and DSBA recombinant antigen fragments using DSBA antibody (1:1000 diluted).| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol. |
