Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Dynamin 2 antibody at 1:500 dilution.Application
Sample(30 ug whole cell lysate) A:MOLT4 B:Raji 7.5% SDS PAGE diluted at 1:1000Application
Dynamin 2 antibody [C2C3], C-term detects DNM2 protein at cytoplasm by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DNM2 protein stained by Dynamin 2 antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33343 staining. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]| Product Name | Dynamin 2 antibody , C-term |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Dynamin 2 (dynamin 2)Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Four alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Dynamin 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CMT2M antibody CMTDI1 antibody CMTDIB antibody DI-CMTB antibody DYN2 antibody DYNII antibody DNM2 antibody dynamin II antibody dynamin-2 antibody dynamin 2 antibody |
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| Molecular Weight(MW) | 98 kDa (note) (5) |
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Dynamin 2 antibody at 1:500 dilution.
Application
Sample(30 ug whole cell lysate) A:MOLT4 B:Raji 7.5% SDS PAGE diluted at 1:1000
Application
Dynamin 2 antibody [C2C3], C-term detects DNM2 protein at cytoplasm by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DNM2 protein stained by Dynamin 2 antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33343 staining. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
