Application
Immunofluorescence analysis of methanol-fixed HeLa, using Dynamin 2 antibody at 1:500 dilution.Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Dynamin 2 antibody [N1N3] detects Dynamin 2 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Dynamin 2 antibody [N1N3] dilution: 1:500.Application
Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:5000| Product Name | Dynamin 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Dynamin 2 (dynamin 2)Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Four alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Dynamin 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CMT2M antibody CMTDI1 antibody CMTDIB antibody DI-CMTB antibody DYN2 antibody DYNII antibody DNM2 antibody dynamin II antibody dynamin-2 antibody dynamin 2 antibody |
|---|---|
| Molecular Weight(MW) | 98 kDa (note) (6) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Dynamin 2 antibody at 1:500 dilution.
Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Dynamin 2 antibody [N1N3] detects Dynamin 2 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Dynamin 2 antibody [N1N3] dilution: 1:500.
Application
Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:5000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
