| Product Name | Dynein Intermediate chain antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [70.1] to Dynein intermediate chainEukaryotic cells rely on actin and microtubule-based protein ""motors"" to generate intracellular movements (4). These protein ""motors"" contain specialized domains that hydrolyze ATP to produce force and movement along a cytoskeletal polymer (actin in the case of myosin family and microtubules in the case of the kinesin family and dyneins). Dynein has been implicated in cytoplasmic motile functions, including chromosomal movement, retrograde organelle and axonal transport, the endocytic pathway, and the organization of the Golgi apparatus. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (~450 kD), three intermediate chains (70-125 kD), and at least four light chains (15-25 kD) (5). |
| Immunogen | Cytoplasmic full length native protein (purified from chick brain) (Chicken). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Host Species | Mouse |
| Tested Applications | DotELISAICC/IFIPPurifyWB |
| For Dot: Use at an assay dependant dilution. For ELISA: Use at an assay dependant dilution. For ICC: Use at an assay dependant dilution. For IF: Use at an assay dependent dilution. For IP: Use at an assay dependant dilution. For WB: Use at a dilution of 1:2,000 using chicken brain extract. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanRatHamsterIndian Muntjac |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DNAI1 ICS MGC26204 242650 Dynein Intermediate chain PCD Q9UI46 CILD1 27019 |
|---|---|
| Target | Dynein Intermediate chain |
| Tissue Specificity | This antibody identifies the cytoplasmic dynein intermediate chains (triplet of approximately 70 kD) of all vertebrates tested, in immunoblotting with dynein enriched preparations and with crude cell extracts. |
| Cellular Localization | Cytoplasmic |
| Application Notes | For Dot: Use at an assay dependant dilution. For ELISA: Use at an assay dependant dilution. For ICC: Use at an assay dependant dilution. For IF: Use at an assay dependent dilution. For IP: Use at an assay dependant dilution. For WB: Use at a dilution of 1:2,000 using chicken brain extract. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Ascitic fluid containing 15 mM sodium azide |
