| Product Name | E. coli SLT-1 + SLT-2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [286-13310] to E. coli SLT-1 + SLT-2 |
| Immunogen | SLT Toxin from E. coli O157:H7. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 286-13310 |
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| Toxin Neutralization: Neutralized Vero Cell toxicity of E. coli O157:H7 extracts when used at a 10 μg/mL concentration. Immunoblot: Detected SLT 1 when used at 1 μg/mL concentration. ELISA: Used as capture antibody at 20 μg/mL concentration (100μL). Immobilizes SLT 1 for detection by an HRP conjugate. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.: |
|
| Species Reactivity | E.coli |
| Concentration | 1mg/ml |
| Target | E. coli SLT-1 + SLT-2 |
|---|---|
| Tissue Specificity | Reacts with alpha, alpha-1 and beta subunits of SLT-1. Also reacts with alpha and alpha-1 subunits of SLT-2. |
| Application Notes | Toxin Neutralization: Neutralized Vero Cell toxicity of E. coli O157:H7 extracts when used at a 10 μg/mL concentration. Immunoblot: Detected SLT 1 when used at 1 μg/mL concentration. ELISA: Used as capture antibody at 20 μg/mL concentration (100μL). Immobilizes SLT 1 for detection by an HRP conjugate. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 10 mM Phosphate containing 0.9% Sodium Chloride, pH 7.4. |
