Application
Western blot analysis of extracts from Mouse brain tissue using E2F1 (Phospho-Thr433) Antibody. The lane on the left is treated with the antigen-specific peptide.| Product Name | E2F1 (phospho Thr433) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to E2F1 (phospho Thr433)The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008] |
| Immunogen | Peptide sequence around phosphorylation site of Threonine 433 (D-L-T(p)-P-L) derived from Human E2F1. |
| Modification | p-Thr433 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | RBBP3 E2F-1 RBP3 E2F transcription factor 1 RBAP1 E2F1 |
|---|---|
| Target | E2F1 (phospho Thr433) |
| Tissue Specificity | The antibody detects endogenous level of E2F1 only when phosphorylated at Threonine 433. |
Application
Western blot analysis of extracts from Mouse brain tissue using E2F1 (Phospho-Thr433) Antibody. The lane on the left is treated with the antigen-specific peptide.| Application Notes | WB: 1:500 - 1:1000: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
