Application
Western blot analysis of E2F1 (pT433) expression in Jurkat colchicine-treated (A); mouse spleen (B); PC12 colchicine-treated (C) whole cell lysates.| Product Name | E2F1 (phospho Thr433) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to E2F1 (phospho Thr433)The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human E2F1. The exact sequence is proprietary. |
| Modification | p-Thr433 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | E2F transcription factor 1 RBBP3 RBAP1 E2F1 E2F-1 RBP3 |
|---|---|
| Target | E2F1 (phospho Thr433) |
| Tissue Specificity | Recognizes endogenous levels of E2F1 (pT433) protein. |
Application
Western blot analysis of E2F1 (pT433) expression in Jurkat colchicine-treated (A); mouse spleen (B); PC12 colchicine-treated (C) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
