Application
IHC-P analysis of esophageal cancer tissues using E2F1 antibody [8G9].Application
ELISA analysis using E2F1 antibody [8G9] and either control antigen or immunogen antigen.Application
FACS analysis of HeLa cells using E2F1 antibody [8G9] (green) and negative control (red).Application
WB analysis of (1) HeLa, (2) SK-N-SH and (3) NIH3T3 lysates using E2F1 antibody [8G9].| Product Name | E2F1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [8G9] to E2F1The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 200 |
| Immunogen | The immunogen corresponding to a region within amino acids 69 and 223 of human E2F1 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 8G9 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | E2F-1 RBBP3 RBP3 RBAP1 |
|---|---|
| Target | E2F1 |
| Molecular Weight(MW) | 46.9 kDa (note) |
Application
IHC-P analysis of esophageal cancer tissues using E2F1 antibody [8G9].
Application
ELISA analysis using E2F1 antibody [8G9] and either control antigen or immunogen antigen.
Application
FACS analysis of HeLa cells using E2F1 antibody [8G9] (green) and negative control (red).
Application
WB analysis of (1) HeLa, (2) SK-N-SH and (3) NIH3T3 lysates using E2F1 antibody [8G9].| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer. |
