Rabbit polyclonal to EB3EB3 is a 282 amino acid homologue of EB1, a protein known to bind Adenomatous Polyposis Coli. It has been shown to bind to tubulin in vitro and microtubules in vivo. Northern blotting shows expressions to be mainly in brain, and to a lesser degree in muscle. EB3 interacts with APCL, a homologue of the tumor suppressor APC, and this interaction may be involved in the stabilization or extension of microtubules.
Immunogen
Synthetic peptide (Human).
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
WB: Use at a concentration of 0.5 - 2 μg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa). Optimal dilutions/concentrations should be determined by the end user.:
Western blot analysis of EB3 using on 15 ugs of human brain lysate.
Positive Control
Human brain whole cell lysate
Application Notes
WB: Use at a concentration of 0.5 - 2 μg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa). Optimal dilutions/concentrations should be determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.