Application
Immunoprecipitation of E-Cadherin (phospho Ser838/840) protein from MCF-7 whole cell extracts using 5 μg of E-Cadherin (phospho Ser838/840) antibody . Western blot analysis was performed using E-Cadherin (phospho Ser838/840) antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
E-Cadherin (phospho Ser838/840) antibody detects E-Cadherin (phospho Ser838/840) protein by western blot analysis. MCF-7 whole cell extract (30 μg) with the indicated treatments were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody at a dilution of 1:10000.Application
E-Cadherin (phospho Ser838/840) antibody detects E-Cadherin (phospho Ser838/840) protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody diluted at 1:10000.| Product Name | E-Cadherin (phospho Ser838/840) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to E-Cadherin (phospho Ser838/840) (cadherin 1, type 1, E-cadherin (epithelial))This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary. |
| Modification | p-Ser838/840 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Xenopus laevis, Dog, PigMouse (100%), Xenopus laevis (100%), Dog (100%), Pig (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | E-Cadherin phospho Ser838 Ser840 ECAD phospho Ser838 Ser840 E-Cadherin CDHE CDH1 E-Cadherin phospho S838 S840 E-Cadherin (pS838 pS840) E-Cadherin (pSer838 pSer840) phospho E-Cadherin (S838 S840) E-Cadherin phospho E-Cadh |
|---|---|
| Target | E-Cadherin (phospho Ser838/840) |
| Molecular Weight(MW) | 97 kDa (note) (2) |
| Cellular Localization | Cell junction , Cell membrane |
Application
Immunoprecipitation of E-Cadherin (phospho Ser838/840) protein from MCF-7 whole cell extracts using 5 μg of E-Cadherin (phospho Ser838/840) antibody . Western blot analysis was performed using E-Cadherin (phospho Ser838/840) antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
E-Cadherin (phospho Ser838/840) antibody detects E-Cadherin (phospho Ser838/840) protein by western blot analysis. MCF-7 whole cell extract (30 μg) with the indicated treatments were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody at a dilution of 1:10000.
Application
E-Cadherin (phospho Ser838/840) antibody detects E-Cadherin (phospho Ser838/840) protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody diluted at 1:10000.| Positive Control | MCF-7 , MCF-7 CIP , PI , Rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
