WB
Western blot analysis of lysates from HeLa cells, using Phospho-E-Cadherin-S838/S840 Rabbit pAb at 1:1000 dilution. HeLa cells were treated with Pervanadate (1 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.| Product Name | Phospho-E-Cadherin-S838/S840 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Modification | p-S838/S840 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500 - 1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDH1 |
|---|---|
| Gene Synonyms | UVO CDHE ECAD LCAM Arc-1 BCDS1 CD324 |
| Gene Full Name | cadherin 1 |
| Gene Summary | This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015] |
| Alternative Names | UVO CDHE ECAD LCAM Arc-1 BCDS1 CD324 Phospho-E-Cadherin-S838/S840 |
| Molecular Weight(MW) | 97kDa(Observed band size: 125kDa) |
| Cellular Localization | Cell junction, Cell membrane, Endosome, Golgi apparatus, Single-pass type I membrane protein, trans-Golgi network. |
WB
Western blot analysis of lysates from HeLa cells, using Phospho-E-Cadherin-S838/S840 Rabbit pAb at 1:1000 dilution. HeLa cells were treated with Pervanadate (1 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.| Application Notes | WB:1:500 - 1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
