WB
Western blot analysis of extracts from HepG2, U-2 OS, U251, LNCaP cells using it at 1:1000.IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer using it antibody.| Product Name | Phospho-E-Cadherin (Ser838/Ser840) Recombinant Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser838/Ser840 of human E-Cadherin. |
| Modification | p-Ser838/Ser840 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:5000 IHC:1:5000-1:50000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDH1 |
|---|---|
| Gene Synonyms | UVO CDHE ECAD LCAM Arc-1 BCDS1 CD324 |
| Gene Full Name | cadherin 1 |
| Gene Summary | This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015] |
| Alternative Names | UVO CDHE ECAD LCAM Arc-1 BCDS1 CD324 |
| Molecular Weight(MW) | 135 kDa |
| Cellular Localization | Cell junction,Cell membrane,Cytoplasm,Endosome,Golgi apparatus,Membrane,Secreted. |
WB
Western blot analysis of extracts from HepG2, U-2 OS, U251, LNCaP cells using it at 1:1000.
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer using it antibody.| Application Notes | WB:1:1000-1:5000 IHC:1:5000-1:50000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 1mg/ml in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
