Application
E-cadherin antibody [GT311] detects E-cadherin protein by Western blot analysis. A. 30 μg MCF-7 whole cell lysate/extract B. 30 μg MDA-MB-231 whole cell lysate/extract 5 % SDS-PAGE E-cadherin antibody [GT311] dilution: 1:1000Application
E-adherin antibody [GT311] immunoprecipitates E-adherin protein in IP experiments. IP samples: MCF-7 whole cell extract A. 30 μg MCF-7 cell whole cell extract B. Control with 3 μg of preimmune Mouse IgG C. Immunoprecipitation of E-adherin protein by 3 μg E-adherin antibody [GT311] 5 % SDS-PAGE The immunoprecipitated E-adherin protein was detected by E-adherin antibody [GT311] diluted at 1:500. [EasyBlot anti-mouse IgG was used as a secondary reagent]| Product Name | E-Cadherin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT311] to E-Cadherin (cadherin 1, type 1, E-cadherin (epithelial))This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT311 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, BovineMouse (80%), Rat (83%), Dog (80%), Pig (83%), Bovine (82%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | E-cadherin |
|---|---|
| Molecular Weight(MW) | 97 kDa (note) (2) |
| Cellular Localization | Cell junction , Cell membrane |
Application
E-cadherin antibody [GT311] detects E-cadherin protein by Western blot analysis. A. 30 μg MCF-7 whole cell lysate/extract B. 30 μg MDA-MB-231 whole cell lysate/extract 5 % SDS-PAGE E-cadherin antibody [GT311] dilution: 1:1000
Application
E-adherin antibody [GT311] immunoprecipitates E-adherin protein in IP experiments. IP samples: MCF-7 whole cell extract A. 30 μg MCF-7 cell whole cell extract B. Control with 3 μg of preimmune Mouse IgG C. Immunoprecipitation of E-adherin protein by 3 μg E-adherin antibody [GT311] 5 % SDS-PAGE The immunoprecipitated E-adherin protein was detected by E-adherin antibody [GT311] diluted at 1:500. [EasyBlot anti-mouse IgG was used as a secondary reagent]| Positive Control | MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
