Application
ICC/IF analysis of human HDFn cells using ECH1 antibody [9D11AF3] at a concentration of 4 μg/ml (green) and DAPI (blue).| Product Name | ECH1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [9D11AF3] to ECH1This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq, Jul 2008] |
| Immunogen | ECH1 contains a C-terminal peroxisomal targeting sequence and localizes to the peroxisome |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 9D11AF3 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIP |
| ICC/IF:1:200~1:500 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | enoyl CoA hydratase 1 peroxisomal HPXEL |
|---|---|
| Target | ECH1 |
Application
ICC/IF analysis of human HDFn cells using ECH1 antibody [9D11AF3] at a concentration of 4 μg/ml (green) and DAPI (blue).| Application Notes | ICC/IF:1:200~1:500 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4° C |
| Storage Buffer | HEPES buffered saline with 0.02% sodium azide |
